Biotin his-tag
WebJun 2, 2024 · word-forming element in chemistry, usually indicating a neutral substance, antibiotic, vitamin, or hormone; a modification and specialized use of -ine (2). WebKeywords: His-tagged protein, Ni-NTA, biolayer interferometry, avidin-biotin, norovirus, non-labelled detection 1. Introduction Histidine tags, e.g. insertion of a stretch of 5-10 histidine amino acids in either the N- or C-terminus of a recombinant protein, are typically used as affinity tags for purification purposes [1-3].
Biotin his-tag
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WebMouse monoclonal 6X His tag® antibody [AD1.1.10] conjugated to Biotin. Validated in WB, ELISA and tested in Species independent. Immunogen corresponding to synthetic peptide. WebHis-tag is one of the most widely applied tags for recombinant protein expression and purification, and also used for binding assay to detect protein-protein interactions by a …
WebCys-Streptavidin is a recombinant streptavidin for site-specific conjugation. Like the native streptavidin, Cys-Streptavidin has four identical subunits and binds specifically to biotin with an extremely high affinity. However, the subunit of the Cys-Streptavidin protein has a single cysteine residue at N-terminus for direct conjugation. WebBiotin-labeled anti-His-tag antibodies are used to indirectly label proteins tagged with 6XHis. This can be advantageous when working with partially purified protein, or in cases …
WebAnti-Histidine Tagged Antibody, clone 4D11, biotin conjugate. Compare Product No. Clonality Application Species Reactivity; 16-200: 4D11, monoclonal: WB: 5: Expand WebProduct Details. Monoclonal mouse Anti·His Antibodies (5xHis antibodies and 4xHis antibodies), like the RGS·His Antibody, BSA-free, Tetra·His Antibody, BSA-free and Penta·His Antibody, BSA-free are used to detect recombinant protein carrying His tags. Each of the three variants detects a different His tag epitope.
WebRecombinant Human MuSK Avi-tag His-tag Biotin Protein, CF. Cat # AVI10189. Human TfR2 Alexa Fluor® 647-conjugated Antibody. Cat # FAB31202R. Recombinant S. pyogenes CRISPR-associated Protein 9, CF. Cat # 9957-C9. Recombinant Human B7-2/CD86 Fc Chimera Avi-tag, CF. Cat # AVI7625.
WebNov 15, 2007 · We have developed an approach for site-specific reversible biotinylation of recombinant proteins through their histidine tag using biotin conjugated to the multivalent chelator trisnitrilotriacetic acid (BTtris-NTA). Stable binding of BTtris-NTA to His-tagged proteins was demonstrated, which is readily reversed by addition of imidazole ... how many seeds to plant in starter potWebIn a functional flow cytometry test, (A) Recombinant SARS-CoV-2 Spike His-tag Biotin Protein (Catalog # BT10549; 500 ng/ml) binds to HEK293 human embryonic kidney cell line transfected with recombinant human … how many seers is battle axe worth mm2WebCommonly modified sequences, or tags include 6xHIS-tag and FLAG ®-tag, or the addition of GST, MBP, and SUMO domains. The addition of biotin and biotinylated substances to … how many seers is battle axe worthWebThe polyhistidine- or His6-tag is a protein tag originally developed for efficient protein purification in 1988 (Hochuli, Bannwarth & Döbeli et al., 1988). As a result of its comparably great cost-benefit ratio, it is known as one of the most widely used protein affinity tags to date. Due to its small size, variable between four to ten amino ... how many seers is blood worth mm2WebMonoclonal 6-His antibody was raised against a recombinant protein containing the sequence HHHHHH. Phosphate-buffered solution + 0.03% Thimerosal + 50% Glycerol. … how did hernan cortes become famousWebBiotinylated-His peptide, to be used as a probe, assay control, or QC control in Alpha (AlphaLISA®, AlphaScreen®, or AlphaPlex™) no-wash assays. This product contains 0.5 mL of biotinylated-His at 5 µM. This probe can … how many seers is chroma luger worthhow did hernán cortés affect the aztec empire