WebMaiyun, Y., et al. (2014). Biocompatible click chemistry enabled compartment-specific pH measurement inside E. coli.Nature Communications.5: 4981.[Wang, W., et al ... WebBTTAA is a next-generation, water-soluble ligand for the copper (I)-catalyzed azide-alkyne cycloaddition (CuAAC) that dramatically accelerates reaction rates and suppresses cell …

Mapping spatial transcriptome with light-activated proximity

http://www.duofluor.com/product/bttaa/ WebBTTAA concentration optimization In this work, the concentration of BTTAA was optimized for the click reaction. In the procedure of click reaction, a series of BTTAA (0 mM, 0.5 mM, 1 mM, 2 mM, 4 mM, 8 mM) was added in mixture with gently shaking overnight respectively (the alkyne-oligonucleotides carried biotin group on 5’). And then, we ... sawtooth spectrum

Bttaa Jena Bioscience Bioz

WebCopper-catalyzed click reaction on/in live cells† Siheng Li,a Lin Wang,ab Fei Yu,a Zhiling Zhu,a Dema Shobaki,a Haoqing Chen,a Mu Wang,a Jun Wang,a Guoting Qin,a Uriel J. Erasquin,a Li Ren,b Yingjun Wangb and Chengzhi Cai*a We demonstrated that the copper-catalyzed azide–alkyne cycloaddition (CuAAC) reaction could be WebPrepare the CuSO 4:BTTAA-Premix freshly for each experiment. Allow all solutions to warm up to room temperature. Mix the appropriate amount of 100 mM CuSO 4 and 50 mM BTTAA stock solution (Tab. 4) by vortexing and spin down briefly. 44 μl CuSO 4:BTTAA-Premix (1 Assay) is sufficient for the preparation of 500 μl CLICK reaction assay (see. 3.2). WebBTTAA provides greater rate enhancement than the previous generation ligands THPTA or TBTA. It is the latest generation of water-soluble accelerating ligands for CuAAC, and … scala match case