WebFeb 1, 2005 · Polyubiquitin Controlled Synthesis of Polyubiquitin Chains February 2005 DOI: 10.1016/S0076-6879 (05)99002-2 Source PubMed Authors: Cecile M Pickart … WebMar 16, 2024 · In this chapter, we present a collection of protocols for generating monoubiquitin and K63-linked polyubiquitin chains that can be used in pull-down assays to study ubiquitin-binding proteins. The first protocol (Subheading 3.1) describes how to express and purify monoubiquitin from E. coli cells using acid precipitation [ 6 ].
Ubiquitin chains bearing genetically encoded photo-crosslinkers …
WebCircular dichroism and crystallographic analyses are used to verify the structures of the well-folded, synthetic polyubiquitin chains. The facile synthetic strategy is expected to be generally applicable for the rapid synthesis of isopeptide-linked isoUbs and to pave the way for the study of longer polyubiquitin chains. WebFeb 1, 2011 · Methods for polyubiquitin chain synthesis have primarily focused on generating free polyubiquitin chains either enzymatically 16, 17 or chemically 18 . Nevertheless, three main classes of ... moto thouars 79
Controlled Synthesis of Polyubiquitin Chains Request …
WebJan 14, 2024 · Ubiquitin modifications control a plethora of vital cellular processes through proteolytic and nonproteolytic mechanisms, including proteasomal degradation and … WebE2 enzymes catalyze the ATP-dependent polymerization of polyubiquitin chains which function as molecular signals in the regulation of numerous cellular processes. Here we … WebJan 1, 2024 · The protocol below details the analysis of polyubiquitin chains, ubiquitinated proteins and rubylated proteins by top-down tandem MS. The strategy has been established utilizing 5 synthetic ubiquitin conjugates, 3 rubylated conjugates and 20 synthetic ubiquitin chains (7 dimers, 6 trimers, 6 tetramers and 1 pentamer). healthy kids initiative