Nucblue staining
Web29 mei 2024 · Neutrophils were stained with GreenGlo™ Safe DNA Dye (1/5000) for 1 min or NucBlue® (1/1000) for 20 min at room temperature and imaged. In some experiments, cells were fixed and/or permeabilized before or after staining using 4% paraformaldehyde (PFA) and 1 × BD Perm/Wash™ buffer. Web7 feb. 2024 · The cells were then stained with PE-conjugated anti-Cd11b, FITC-conjugated anti-Ly6G, or corresponding isotypes (4 μg/mL for each antibody; BD Biosciences) for 45 min on ice while protected from light. Finally, ... 80 μL of NucBlue reagent and 10 μL of opsonized pHrodo E. coli bioparticles (Molecular Probes, Eugene, OR, USA).
Nucblue staining
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Web5 sep. 2016 · BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometry Lipid droplets (LDs) are ubiquitous, dynamic organelles and function as a storage depot for neutral lipids, including triglycerides and cholesterol esters (Walther and Farese, 2012). WebProtocols used specific antibodies as described previously: 23 actin green (Molecular Probes R37110, excitation 488nm/emission 500–550nm), anti-IL8 1/1500 (Abcam Ab18672), anti-p16INK4a 1/150 (Abcam Ab192053), alexa fluor 594-coupled secondary antibody 1/1000 (Life Technologies A11020, excitation 561nm/emission 570–620nm) …
Web9 mrt. 2015 · Optional: You can stain the cells with NucBlue™ Live Cell Stain, a nuclear counterstain, or another counterstain at this time. 6. Optional: If you are using the CellROX® Green Reagent, you can permeabilize the cells with 0.5% Triton® X-100 for 10 minutes, if cell permeabilization is required for multiplexing with another reagent. Web(Scale bar: 10 mm.) (C) Whole-mount immunofluorescence staining of the 3D tissue, in which Na+/K ATPase, CD31, and nuclei (NucBlue staining) are denoted by green, red, and blue, respectively. (Scale bar: 1 mm.) Note: The separation distance between the PT and vascular conduits is ∼70 μm. (Inset) Cross-sectional images of the two open lumens.
WebNucBlue is simply Hoechst 33342, which is a good DNA stain that is very stable and compatible with extended duration live cell imaging. However, because Hoechst is … Webtimes between 5 and 30 minutes are typical for bright nuclear stain - ing, though some optimization may be required for certain cell types and growth conditions. Washing steps are not usually necessary; however, staining intensity will increase with time if cells are not washed prior to imaging. NucBlue ®
WebIn most cases 2 drops/mL and an incubation of 15 to 30 minutes will produce bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications Excited by UV light at 360nm when bound to DNA, with an emission maximum at 460nm It is detected through blue/cyan filter
Web14 apr. 2024 · The activation of P2X7 is a well-known stimulus for the NLRP3-caspase 1 inflammasome and subsequent rapid IL-1β secretion from monocytes and macrophages. Here we show that positive allosteric modulators of P2X7, ginsenosides, can enhance the release of three important cytokines, IL-1β, IL-6 and TNF-α from LPS-primed rodent … scullery workWeb30 sep. 2024 · (2)Indirect DNA stain using indicator cells e.g. 3T3 or 3T6. In this analysis, the test culture supernatant is added to a mycoplasma-free indicator cell culture (lines Vero B4, NIH 3T3 or 3T6). Cells are grown in flasks containing sterile slips, which are washed and stained with fluorescents after several days of cell culture growth. pdf exceed sizeWebNucBlue Fixed Cell ReadyProbes Reagent is a high-purity formulation of the classic stain DAPI. The room temperature-stable solution is provided in a convenient-to-use dropper … scullery workers crossword